L-Aspartic acid α-(7-amido-4-methyl)coumarin
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L-Aspartic acid α-(7-amido-4-methyl)coumarin

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Category
β−Amino Acids
Catalog number
BAT-005855
CAS number
219138-13-3
Molecular Formula
C14H14N2O5
Molecular Weight
290.27
L-Aspartic acid α-(7-amido-4-methyl)coumarin
IUPAC Name
(3S)-3-amino-4-[(4-methyl-2-oxochromen-7-yl)amino]-4-oxobutanoic acid
Synonyms
H-Asp-AMC
Storage
Store at 2-8°C
InChI
InChI=1S/C14H14N2O5/c1-7-4-13(19)21-11-5-8(2-3-9(7)11)16-14(20)10(15)6-12(17)18/h2-5,10H,6,15H2,1H3,(H,16,20)(H,17,18)/t10-/m0/s1
InChI Key
AAGPGZSFBVEQMU-JTQLQIEISA-N
Canonical SMILES
CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CC(=O)O)N

L-Aspartic acid α-(7-amido-4-methyl)coumarin, a synthetic compound with diverse applications in biosciences, plays a pivotal role in various scientific endeavors. Here are four key applications:

Enzyme Activity Assays: Serving as a fluorogenic substrate in enzyme activity assays, L-Aspartic acid α-(7-amido-4-methyl)coumarin undergoes hydrolysis by specific enzymes, unleashing a fluorescent product for easy quantification. This mechanism enables researchers to meticulously gauge enzyme kinetics and inhibition with exceptional sensitivity and precision.

Drug Screening: Positioned as a valuable asset in high-throughput drug screening platforms, this compound facilitates swift identification of potential inhibitors or activators of target enzymes in pharmaceutical assays. By integrating L-Aspartic acid α-(7-amido-4-methyl)coumarin, researchers can expedite the process of drug discovery by pinpointing robust drug candidates effectively and efficiently.

Biomedical Research: Encompassing studies on protein-protein interactions, L-Aspartic acid α-(7-amido-4-methyl)coumarin assumes a pivotal role as a reporter for binding events, unravelling complex biological pathways. This application stands as a cornerstone in deciphering disease mechanisms and uncovering novel therapeutic targets, shedding light on intricate biological processes with unprecedented depth.

Diagnostics: In clinical diagnostics, L-Aspartic acid α-(7-amido-4-methyl)coumarin emerges as a potent tool in assays designed to detect enzyme deficiencies or anomalies in patient specimens. The fluorescent signal generated offers a lucid and quantifiable output, streamlining the diagnosis of metabolic disorders and other health conditions with enhanced precision and efficacy.

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