1.Natural killer cells inhibit pulmonary metastasis of hepatocellular carcinoma in nude mice.
Hong ZF1, Zhao WX2, Yin ZY2, Xie CR2, Xu YP2, Chi XQ2, Zhang S2, Wang XM2. Oncol Lett. 2016 Mar;11(3):2019-2026. Epub 2016 Feb 1.
Natural killer (NK) cells have been demonstrated to inhibit tumor growth. However, the role of NK cells in the inhibition of hepatocellular carcinoma metastasis is not well understood. The present study aimed to investigate the roles that NK cells may serve in inhibiting hepatocellular carcinoma metastasis. The role of isolated NK cells in the inhibition, proliferation, migration and invasion of the hepatoma cell line, MHCC97-H, was examined in vitro. Additionally, the survival rate of NK cells labeled with carboxyfluorescein diacetate-succinimidyl ester was assessed in vivo. An orthotopic implantation model was used to evaluate the role of NK cells in suppressing MHCC97-H cells in vivo. The effect of interleukin (IL)-2 stimulation on the tumor-inhibitory role of the NK cells was measured indirectly by analyzing the expression of various NK cell receptors and activated NK cell markers. It was observed that the NK cells inhibited the proliferation, migration and invasion of the MHCC97-H cells in vitro.
2.Growth suppression effect of human mesenchymal stem cells from bone marrow, adipose tissue, and Wharton's jelly of umbilical cord on PBMCs.
Ayatollahi M1, Talaei-Khozani T2, Razmkhah M3. Iran J Basic Med Sci. 2016 Feb;19(2):145-53.
OBJECTIVES: Immunosuppressive property of mesenchymal stem cells (MSCs) has great attraction in regenerative medicine especially when dealing with tissue damage involving immune reactions. The most attractive tissue sources of MSCs used in clinical applications are bone marrow (BM), adipose tissue (AT), and Wharton's jelly (WJ) of human umbilical cord. The current study has compared immunomodulatory properties of human BM, AT, and WJ-MSCs.
3.Optimization, Production and Characterization of a CpG-Oligonucleotide-Ficoll Conjugate Nanoparticle Adjuvant for Enhanced Immunogenicity of Anthrax Protective Antigen.
Milley B, Kiwan R, Ott GS, Calacsan C, Kachura M, Campbell JD, Kanzler H, Coffman RL. Bioconjug Chem. 2016 Apr 13. [Epub ahead of print]
We have synthesized and characterized a novel phosphorothioate CpG oligodeoxynucleotide (CpG ODN)-Ficoll conjugated nanoparticulate adjuvant, termed DV230-Ficoll. This adjuvant was constructed from an amine-functionalized-Ficoll, a heterobifunctional linker (succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol] ester) and the CpG-ODN DV230. Herein, we describe the evaluation of the purity and reactivity of linkers of different length for CpG-ODN-Ficoll conjugation, optimization of linker coupling and conjugation of thiol-functionalized CpG to maleimide-functionalized Ficoll and process scale-up. Physicochemical characterization of independently produced lots of DV230-Ficoll reveal a bioconjugate with a particle size of approximately 50 nanometers and covalent attachment of more than 100 molecules of CpG per Ficoll. Solutions of purified DV230-Ficoll were stable for at least 12 months at frozen and refrigerated temperatures and stability was further enhanced in lyophilized form.
4.[Endomorphine-1 inhibits maturation and functions of human peripheral blood-derived dendritic cells].
Yang L1, Wang Y1, Pan Z1, Chen Y2, Xia H2, Li Z3. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Apr;32(4):527-31.
Objective To investigate the effects of endomorphine-1 (EM-1) on the functional features of lipopolysaccharide (LPS)-stimulated peripheral blood-derived dendritic cells (PBDCs), including PBDC phenotypes, cytokine profile and its capability of promoting T cell proliferation. Methods PBDCs were isolated from peripheral blood and differentiated in the presence of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and interleukin-4 (rhIL-4). Then the cells were further activated by LPS or EM-1. Several surface markers were detected by flow cytometry including CD80, CD86, CD83, HLA-DR and CCR7. ELISA was used to detect cytokine levels of IL-10, IL-12, IFN-γ in the supernatants of the cultured PBDCs. Using co-culture of T lymphocytes and PBDCs stimulated by EM-1, the proliferation of T cells induced by PBDCs was determined by 5, 6-carboxyfluorescein succinimidyl ester (CFSE) staining. Results EM-1 down-regulated the expressions of CD80, CD86, CD83, HLA-DR and CCR7 on the activated PBDCs.
