1.Transgenic Expression of Human Lysophosphatidic Acid Receptor LPA2 in Mouse Intestinal Epithelial Cells Induces Intestinal Dysplasia.
Yoshida M1,2, He P1, Yun CC1,3. PLoS One. 2016 Apr 28;11(4):e0154527. doi: 10.1371/journal.pone.0154527.
Lysophosphatidic acid (LPA) acts on LPA2 receptor to mediate multiple pathological effects that are associated with tumorigenesis. The absence of LPA2 attenuates tumor progression in rodent models of colorectal cancer, but whether overexpression of LPA2 alone can lead to malignant transformation in the intestinal tract has not been studied. In this study, we expressed human LPA2 in intestinal epithelial cells (IECs) under control of the villin promoter. Less than 4% of F1-generation mice had germline transmission of transgenic (TG) human LPA2; as such only 3 F1 mice out of 72 genotyped had TG expression. These TG mice appeared anemic with hematochezia and died shortly after birth. TG mice were smaller in size compared with the wild type mouse of the same age and sex. Morphological analysis showed that TG LPA2 colon had hyper-proliferation of IECs resulting in increased colonic crypt depth. Surprisingly, TG small intestine had villus blunting and decreased IEC proliferation and dysplasia.
2.Sensitivity of HIV rapid tests compared to fourth generation enzyme immunoassays or HIV RNA tests - a systematic review and meta-analysis.
Tan WS1, Chow EP, Fairley CK, Chen MY, Bradshaw CS, Read TR. AIDS. 2016 Apr 27. [Epub ahead of print]
OBJECTIVE: Determine the sensitivity of rapid HIV tests (RT) compared to fourth generation enzyme immunoassays (EIA) or nucleic acid amplification tests (NAAT) in clinical settings.
3.Identification of Factors Affecting the Success of Next-Generation Sequencing Testing in Solid Tumors.
Goswami RS1, Luthra R2, Singh RR1, Patel KP1, Routbort MJ1, Aldape KD3, Yao H4, Dang HD1, Barkoh BA1, Manekia J1, Medeiros LJ1, Roy-Chowdhuri S3, Stewart J3, Broaddus RR3, Chen H3. Am J Clin Pathol. 2016 Feb;145(2):222-237. Epub 2016 Feb 12.
OBJECTIVES: Clinical laboratories are rapidly implementing next-generation sequencing (NGS) tests for mutation analysis, but there are few guidelines regarding sample quality for successful results.
4.MULTIMODAL IMAGING AND HISTOLOGIC CORRELATION OF ISOLATED METASTASIS OF PROSTATE ADENOCARCINOMA TO THE CHOROID.
Cheung AY1, Yonekawa Y, Balaraman S, Thomas BJ, Xi AM, Folberg R, Amin M, Miller KT, Faia LJ. Retin Cases Brief Rep. 2016 Apr 27. [Epub ahead of print]
PURPOSE: To provide correlative clinical-multimodal imaging-histopathologic findings of isolated prostatic choroidal metastasis.
