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CovidyteTM EN450, a peptide substrate, can be cleaved by coronavirus proteases. The dark-FRET peptide contains Dabcyl (quencher) at the N-terminus and Edans (donor) at the C-terminus, respectively, and the fluorescence of Edans is effectively quenched by Dabcyl when the peptide is intact. When it is hydrolyzed by the coronavirus protease, the fluorescence of the Edans fragment is significantly enhanced because its fluorescence is no longer quenched by Dabcyl. Edans fluorescence intensity can effectively monitor the activity of coronavirus protease. It is a handy tool for screening coronavirus protease inhibitors. (excitation at 350, emission at 460 nm)
