Defensin D7

The aim of this study was to determine the changes in the expression of avian β-defensins (AvBDs) and proinflammatory cytokines and localization of AvBD2 in the intestine of broiler embryos and chicks during growth. The ileum and cecum of embryonic day 19 (ED19) and of day-old (D0) and 7-day-old (D7) chicks were collected. Gene expression levels of 10 AvBDs (AvBD1-8, 10, and 12) and proinflammatory cytokines (IL-1β, -6, and -8) were analyzed using real-time PCR, and the localization of AvBD2 was examined by immunohistochemistry. Gene expression levels of AvBD1, 2, 6, and 7 in the ileum and of AvBD1 and 4 in the cecum were higher on ED19 than on D7. The expression of AvBD10 in the ileum was higher on D0 than on ED19, whereas the expression levels of AvBD8 and 10 in the cecum were higher on D0 than on ED19, and that of AvBD10 decreased on D7. The expression levels of IL-1β, -6, and -8 in the ileum were higher on D7 than on ED19. The expression levels of IL-1β, -6, and -8 in the cecum were higher on D0 than on ED19, and that of IL-1β and -6 declined on D7. AvBD2-positive cells were localized in the lamina propria beneath epithelial cells of villi and crypts. The number of positive cells in the cecum mucosa was greater on D0 than on ED19 and D7. In conclusion, we suggest that AvBDs are expressed in the ileum and cecum of embryos and chicks at high levels before or just after hatching and decrease by D7. The expression of proinflammatory cytokines in the ileum increases with growth until D7, but is the highest in the cecum around hatching. These AvBDs and proinflammatory cytokines may play roles in host defense in the intestinal mucosa of embryos and neonatal chicks.

The effects of glutamine (Gln) on immunity and intestinal barrier gene expression levels in broilers challenged with Salmonella Enteritidis were evaluated. A total of 400 1-day-old broilers were randomly assigned to four groups, 10 repetition treatments per group with 10 broiler chickens for a 21-day feeding trial. The groups were the normal control group (CON, no infected group, fed with a basal diet); the S. Enteritidis-infected control group (SCC, infected with 2.0 × 104 CFU/mL of S. Enteritidis, fed a basal diet); and the Gln 1 and 2 groups, who were challenged with S. Enteritidis and fed a basal diet plus Gln at 0.5% and 1.0%, respectively. The results show that S. Enteritidis had adverse effects on the average daily feed intake, average daily gain, and the feed conversion ratio of infected broilers compared with those of CON broilers on d 7 (p < 0.05); decreased serum immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) concentrations, and intestinal mucosa Bcl-2 mRNA expression levels (p < 0.05); increased the Lysozyme (LZM, only serum), NO, inducible NO synthase (iNOS) (except at 4 d), and total nitric oxide synthase (TNOS) (except at 4 d) activities in serum and the intestinal mucosa; and increased intestinal mucosa polymeric immunoglobulin receptor (pIgR) (except at 21 d), Avian beta-defensin 5 (AvBD5), AvBD14, Bax, and Bak mRNA expression levels during the experimental period (p < 0.05). Supplementation with Gln improved growth performance; increased serum IgA, IgG, and IgM concentrations and intestinal mucosa Bcl-2 mRNA expression levels (p < 0.05); decreased the LZM (only serum), NO, iNOS (except at 4 d), and TNOS (except at 4 d) activities in serum and the intestinal mucosa; and decreased intestinal mucosa pIgR (except at 21 d), AvBD5, AvBD14, Bax, and Bak mRNA expression levels during the experimental period (p < 0.05). These results suggest that Gln might lessen the inflammatory reaction of the small intestine and enlarge the small bowel mucosa immune and barrier function in broiler chickens challenged with S. Enteritidis.

Host defense peptides (HDPs) are a large group of small, positively charged peptides that play an important role in innate immunity, particularly at early ages when other components of the immune system have not fully developed. There are 3 classes of avian HDPs: avian beta defensins (AvBDs), cathelicidins (Cath), and liver-expressed antimicrobial peptide 2 (LEAP-2). The objective was to compare expression of HDP mRNAs in male turkey poults at day of hatch (d 0), d 7, d 14, d 21 and d 28 from the thymus, spleen, bursa, duodenum, jejunum, and ileum. The expression of AvBD1, AvBD2, AvBD8, AvBD9, AvBD10, AvBD13, Cath2, Cath3, and LEAP-2 mRNA was measured using qPCR (n = 6 birds/tissue/age). Data were analyzed by one-way ANOVA and Tukey's test, and significance considered at P < 0.05. AvBDs and Caths exhibited greater expression in immune organs (thymus, spleen, and bursa) than intestinal tissues. In the thymus, expression of all AvBDs examined, except AvBD8, showed an increase from d 0 to d 21. In the spleen, AvBD1 and AvBD2 exhibited reduced expression from d 0 to d 7 and low expression thereafter. In the intestine, AVBD1, AVBD8, and AvBD13 increased expression from d 0 to d 28 in the duodenum, while AvBD10 showed the greatest expression at d 0 that declined to d 7 and stayed low thereafter in the duodenum, jejunum, and ileum. Cath2 and Cath3 demonstrated the highest expression in the spleen, which was greatest at d 0 then declined to d 7 through d 28. Conversely, LEAP-2 showed greater expression in the intestinal tissues than in the immune organs. LEAP-2 expression was upregulated from d 0 to d 7 and then remained elevated from d 7 through d 14 in the duodenum. In the jejunum, LEAP-2 increased from d 0 to d 21 and d 28. Understanding the differential expression of HDPs could reveal the innate immune status of turkey poults, and may subsequently allow improvement of their health through appropriate mitigation strategies.