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Pleurain-a1 is an antimicrobial peptide found in Rana pleuraden (Yunnan pond frog, Babina pleuraden), and has antibacterial activity against gram-negative bacteria Escherichia coli, Helicobacter pylori NCTC 11637 (MIC=30 µg/ml) and gram-positive bacteria Bacillus dysenteriae (MIC=120 µg/ml), Staphylococcus aureus, and has anti-yeast activity against Candida albicans.